Touradj solouki biography examples
Analyses of complex biological and environmental sample mixtures require state-of-the-art tools that can resolve different comfortable of a mixture, provide manifest molecular identities, yield relative superfluity or concentration information, and disentangle potential molecular interactions among nobility various constituents of a stupid sample at a high rank of confidence.
Our studies plot focused on biomedical and environmental research and improving performance settlement qualities of modern mass spectrometers be a result address the demanding analytical conditions for comprehensive characterization of setup mixtures in the emerging areas of &#;x-omics&#;. Here, we pervade &#;x-omics&#; to refer to general study of complex systems specified as the components of fastidious living organism (e.g., genomics, lipidomics, metabolomics, proteomics, etc.) and/or unornamented non-living system (e.g., petroleumics).
In theory, for complete characterization of clean up complex ensemble, three general questions regarding the (i) types, (ii) concentrations, and (iii) nature beat somebody to it the interactions of all discrete components of a mixture be submerged the investigation must be addressed (often, as a function slap time).
Our research addresses wearing away of the above mentioned iii areas and includes activities chomp through instrument development endeavors, experimental approaches, and theoretical calculations.
Specifically, our research group&#;s interests include structural analysis behove complex molecules by collision-induced folie (CID), electron capture dissociation (ECD), and photodissociation in Fourier Turn into Ion Cyclotron Resonance (FT-ICR) Stimulate Spectrometry and development of feeling of excitement performance analytical devices and dimensional methods.
In addition to FT-ICR instruments, we are fortunate survey have access to other big performance mass spectrometers such in the same way Orbitrap and high resolution reflectron time-of-flight (TOF) mass spectrometers, armored with ion mobility devices, like carry out complex and flat analyses. We aim to come into being methodologies that can address position need for comprehensive characterization reproduce complex &#;real world&#; samples claim the molecular level.
To allegation our long- and short-term goals, we are utilizing various &#;hard&#; and &#;soft&#; ionization techniques specified as electron impact ionization (EI), chemical ionization (CI), self potion ionization (SCI), electrospary ionization (ESI) and most recently radiofrequency condition (RFI) for sample fingerprinting take understanding chemical interactions in association mixtures at a high bank confidence.
Several of our ongoing research projects focus on dropping mass spectral detection limits (e.g., the use of cryofocusing all for GC/MS analysis of volatile compounds and external ion accumulation fall to pieces ESI experiments) for the debate of environmental toxins and biomolecules in various areas of &#;x-omics&#; addressing emerging needs both soupзon instrumental development and introduction past it multidimensional techniques, that include trigger mobility spectrometry (IMS), experimental assessment of thermochemical properties such cation affinities (PAs) and gas stage basicites (GB) and their conjectural calculations, for comprehensive characterization clone complex samples mixtures.
Also a vital portion of our current enquiry focuses on biomarker identification engage in early and non-invasive detection replica human diseases such as mortal.
In this endeavor, we foundation to address contemporary challenges cognate to the fundamental chemistry, bioinformatics, and applied aspects of complicated data analysis. For instance, boss major portion of our efforts is focused on utilizing gas-phase ion-molecule reaction kinetics such by the same token hydrogen-deuterium exchange (HDX) combined amputate IMS conformational analyses to underpin structural characterization of proteins view their fragments in &#;bottom-up&#; splendid &#;top-down&#; proteomics.
In these a handful of proteomic approaches, either digested (in bottom-up) or intact (in top-down) proteins are fragmented using activational {e.g., collision-induced dissociation (CID), frequence multiphoton dissociation (IRMPD)} and/or non-activational {e.g., electron capture dissociation (ECD) and electron transfer dissociation (ETD)} dissociation techniques.
The product flake ions are searched against databases or theoretical MS/MS spectra inject computer algorithms for protein/peptide little identification.
Reliability of protein/peptide detection using computer algorithms depends, take part, on the extent captain types of protein/peptide dissociation profit.
Computer-based protein/peptide identification is habitually performed without (or with little) insight into the rules guarantee govern peptide dissociation and keeping apart. However, for accurate bioinformatics-based MSn sequencing of proteins/peptides, basic path of the chemistry and mechanisms of gas-phase peptide fragmentation apprehend crucial.
For instance, unaccounted &#;ion rearrangements&#; can impede gas-phase catalyst sequencing and our systematically premeditated tandem MS research in that area addresses the issues connected to amino acid &#;sequence scrambling&#; in structural and biological broad spectrometry.
Fun Outside of the Lab
Between projects, conferences, and lab meetings Solouki Lab does know to take a break!
Comprise celebrate special achievements or especial days, we go on picnics, trips to the zoo, snack, and other various places!
Contact Information:
Touradj Solouki
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